Human CD200R Protein (C-Fc-Avi) was biotinylated in vitro using BirA ligase. SDS-PAGE analysis under reducing (P+) and non-reducing (P-) conditions shows the protein has a purity greater than 95%. A gel shift assay using co-incubation with streptavidin indicates that the biotinylation efficiency of Human CD200R protein exceeds 80%.
Cat #
Size
Price
Quantity
815203
25 ug
$245
815204
100 ug
$595
Product Details
Application
ELISA, BLI
Format
Liquid, Biotinylated
Expression Host
CHO
Target Name
CD200R, CRTR2, MOX2R, OX2R
Species
Human
Sources
Recombinant Human CD200R1 (Ala27-Leu266) with C-terminus Fc-Avi-tag is expressed in CHO cell. This protein was site-specifically labeled with Biotin by BirA ligase.
accession number
Q8TD46
Molecular Weight
The protein has a predicted molecular weight of 55.3 kDa. Under DTT-reducing conditions, it migrates at approximately 80-110 kDa on SDS-PAGE.
Affinity Tag
C-Fc-Avi
Purity
>95% based on SDS-PAGE under reducing condition
Formulation
1xPBS buffer, pH7.4, 0.22 µm filtered
Endotoxin level
Not tested
Protein Concentration
25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and Handling
Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.
Background Information
The cluster of differentiation (CD) system is essential for immunophenotyping, with over 320 unique CD molecules and subclusters identified to date. These CD molecules play various roles in immune cells, either acting as receptors or ligands to initiate signaling cascades that alter cell behavior, or serving non-signaling functions like cell adhesion. CD200 receptor 1 (CD200R1), an isoform of CD200 receptors, is a cell surface glycoprotein expressed on myeloid lineage cells. It serves as a receptor for the OX-2 membrane glycoprotein, and the interaction between CD200R1 and OX-2 functions as a downregulatory signal for myeloid cells, playing a role in immune regulation.
Human CD200R Protein (C-Fc-Avi) was biotinylated in vitro using BirA ligase. SDS-PAGE analysis under reducing (P+) and non-reducing (P-) conditions shows the protein has a purity greater than 95%. A gel shift assay using co-incubation with streptavidin indicates that the biotinylation efficiency of Human CD200R protein exceeds 80%.
