Recombinant Human CD171/L1CAM protein (Ile20-Glu1120) with C-terminus His tag is expressed in CHO cells.
Accession Number
P32004
Molecular Weight
The protein has a predicted molecular weight of 124.7 kDa. Under DTT-reducing conditions, it migrates at approximately 160-200 kDa on SDS-PAGE.
Affinity Tag
C-His
Purity
>95% based on SDS-PAGE under reducing condition
Regulatory Status
RUO
Formulation
1xPBS buffer, pH7.4, 0.22 µm filtered
Endotoxin level
Not tested
Protein Concentration
25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and Handling
Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.
Background Information
CD171, also known as L1CAM or L1, is a 200–220 kD transmembrane glycoprotein and a member of the immunoglobulin superfamily, originally identified for its essential role in nervous system development. It mediates neuron-neuron adhesion, axon guidance, signal transduction, cell migration, and differentiation. Although initially thought to be restricted to neural tissue, L1CAM has since been detected in various non-neural tissues and numerous cancer types. Its expression in tumors is associated with increased cell motility, proliferation, treatment resistance, and poor prognosis, making it a promising target for anti-cancer therapy. Mutations in the L1CAM gene are responsible for the CRASH spectrum of X-linked neurological disorders, including corpus callosum hypoplasia, mental retardation, aphasia, spastic paraplegia, and hydrocephalus. L1CAM interacts with several ligands such as integrins, axonin-1, CD9, and neurocan, with the RGD motif in its sixth Ig domain playing a key role in integrin binding and intracellular signaling.
