Human CD16a F176 Protein (C-His-Avi) was biotinylated in vitro using BirA ligase. SDS-PAGE analysis under reducing (P+) and non-reducing (P−) conditions shows the protein has a purity greater than 95%. A gel shift assay using co-incubation with streptavidin indicates that the biotinylation efficiency of the Human CD16a F176 protein exceeds 80%.
Cat #
Size
Price
Quantity
819303
25 ug
$245
819304
100 ug
$595
Product Details
Application
ELISA, BLI
Format
Liquid, Biotinylated
Expression Host
CHO
Target Name
CD16A, FCGR3A, FCG3, FCGR3, IGFR3
Species
Human
Sources
Recombinant CD16a F176 (Gly17-Gln208) with C-terminus His-Avi-tag is expressed in CHO cell. This protein was site-specifically labeled with Biotin by BirA ligase.
accession number
P08637
Molecular Weight
The protein has a predicted molecular weight of 25.7 kDa. Under DTT-reducing conditions, it migrates at approximately 40 kDa on SDS-PAGE.
Affinity Tag
C-His-Avi
Purity
>95% based on SDS-PAGE under reducing condition
Formulation
1xPBS buffer, pH7.4, 0.22 µm filtered
Endotoxin level
Less than 0.1 EU/µg protein as determined by the LAL method
Protein Concentration
25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and Handling
Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.
Background Information
CD16 is a low-affinity Fc receptor and exists in two isoforms: FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc region of IgG antibodies with distinct tissue-specific expression pattern. CD16a is a transmembrane glycoprotein with intermediate affinity, expressed on natural killer (NK) cells, macrophages, subsets of T cells, immature thymocytes, and placental trophoblasts. In contrast, CD16b is a low-affinity, GPI-anchored receptor expressed primarily on neutrophils and eosinophils. CD16a plays a key role in mediating phagocytosis, antibody-dependent cellular cytotoxicity (ADCC), enzyme secretion, and immune complex clearance.
Human CD16a F176 Protein (C-His-Avi) was biotinylated in vitro using BirA ligase. SDS-PAGE analysis under reducing (P+) and non-reducing (P−) conditions shows the protein has a purity greater than 95%. A gel shift assay using co-incubation with streptavidin indicates that the biotinylation efficiency of the Human CD16a F176 protein exceeds 80%.
