PE conjugated Human CD32a H167 /Fc gamma RIIa (C-His)
Cat #
Size
Price
Quantity
820101
25 ug
$245
820102
100 ug
$595
Product Details
Application
FC
Format
Liquid, PE
Expression Host
CHO
Target Name
CD32a, FCGR2A, CD32, FCG2 , FCGR2A1, IGFR2
Species
Human
Sources
Recombinant CD32a H167 /Fc gamma RIIa (Ala 36 - Ile 218) with C-terminus His-tag is expressed in CHO cell and conjugated to PE.
accession number
Q92835
Molecular Weight
The protein has a predicted molecular weight of 21.9 kDa. Under DTT-reducing conditions, it migrates at approximately 35 kDa on SDS-PAGE prior to conjugation.
Affinity Tag
C-His
Formulation
1xPBS buffer, pH7.4, 0.09% NaN3 with a carrier protein
Endotoxin level
Not tested
Protein Concentration
25µg size is bottled at 0.1mg/mL concentration. 100 µg size is bottled at lot specific concentration.
Storage and Handling
Briefly centrifuge the vial upon receipt. An unopened vial may be stored at 2–8°C for up to six months.
Background Information
FcγRII, or CD32, is a low-affinity receptor for IgG Fc region. In human, it is expressed as three isoforms (A, B, C). The activating isoform, FcγRIIA (CD32a), is found on monocytes, macrophages, granulocytes, platelets, and mast cells. It features an extracellular domain, transmembrane region, and a cytoplasmic tail with a non-classical ITAM, crucial for dimerization and signaling. FcγRIIA plays a key role in inflammation, phagocytosis, and platelet activation, influencing conditions like thrombocytopenia, rheumatoid arthritis, and lupus. Its activity is modulated by lipid rafts, and the receptor’s affinity for IgG subclasses varies. A polymorphism (R167H) impacts FcγRIIA’s binding to IgG2 but not other subclasses
