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Human CD32a R167 /Fc gamma RIIa (C-His)

Human CD32a R167 /Fc gamma RIIa (C-His) on SDS-PAGE under reducing condition (P+) and non-reducing condition (P-). The purity of this protein appears to be greater than 95%.
Human CD32a R167 /Fc gamma RIIa (C-His) on SDS-PAGE under reducing condition (P+) and non-reducing condition (P-). The purity of this protein appears to be greater than 95%.
Cat # Size Price Quantity
82030125 ug$145
820302100 ug$295

Product Details

ApplicationELISA, BLI
FormatLiquid, Purified
Expression HostCHO
Target NameCD32a, FCGR2A, CD32, FCG2 , FCGR2A1, IGFR2
SpeciesHuman
SourcesRecombinant CD32a R167 /Fc gamma RIIa (Ala 36 - Ile 218) with C-terminus His-tag is expressed in CHO cell.
accession numberQ92835
Molecular WeightThe protein has a predicted molecular weight of 21.9 kDa. Under DTT-reducing conditions, it migrates at approximately 35 kDa on SDS-PAGE.
Affinity TagC-His
Purity>95% based on SDS-PAGE under reducing condition
Formulation1xPBS buffer, pH7.4, 0.22 µm filtered
Endotoxin levelLess than 0.1 EU/µg protein as determined by the LAL method
Protein Concentration25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and HandlingBriefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.

Background Information

FcγRII, or CD32, is a low-affinity receptor for IgG Fc region. In human, it is expressed as three isoforms (A, B, C). The activating isoform, FcγRIIA (CD32a), is found on monocytes, macrophages, granulocytes, platelets, and mast cells. It features an extracellular domain, transmembrane region, and a cytoplasmic tail with a non-classical ITAM, crucial for dimerization and signaling. FcγRIIA plays a key role in inflammation, phagocytosis, and platelet activation, influencing conditions like thrombocytopenia, rheumatoid arthritis, and lupus. Its activity is modulated by lipid rafts, and the receptor’s affinity for IgG subclasses varies.