PE conjugated Human CD32b/c /Fc gamma RIIb (C-His)
Cat #
Size
Price
Quantity
820701
25 ug
$245
820702
100 ug
$595
Product Details
Application
FC
Format
Liquid, PE
Expression Host
CHO
Target Name
CD32b/c, FCGR2B, C, FcRII-b, c, CD32, FCG2, IGFR8
Species
Human
Sources
Recombinant CD32b/c /Fc gamma RIIb (Ala46-Pro217) with C-terminus His-tag is expressed in CHO cell and conjugated to PE.
accession number
P31994
Molecular Weight
The protein has a predicted molecular weight of 20.9 kDa. Under DTT-reducing conditions, it migrates at approximately 35 kDa on SDS-PAGE prior to conjugation.
Affinity Tag
C-His
Formulation
1xPBS buffer, pH7.4, 0.09% NaN3 with a carrier protein
Endotoxin level
Not tested
Protein Concentration
25µg size is bottled at 0.1mg/mL concentration. 100 µg size is bottled at lot specific concentration.
Storage and Handling
Briefly centrifuge the vial upon receipt. An unopened vial may be stored at 2–8°C for up to six months.
Background Information
CD32B (FCGR2B) and CD32C (FCGR2C) are 40 kDa, type I transmembrane proteins belonging to the Ig superfamily of low-affinity IgG Fc receptors. CD32B contains an immunoreceptor tyrosine-based inhibition motif (ITIM) in its cytoplasmic tail, while CD32C has an immunoreceptor tyrosine-based activation motif (ITAM). Both receptors can bind monomeric IgG and IgG complexes, and are expressed on B cells, monocytes, macrophages, granulocytes, platelets, and mast cells. CD32B primarily acts as a negative regulator, inhibiting immune cell activation, proliferation, endocytosis, phagocytosis, and degranulation. In contrast, CD32C has an activating function, contributing to immune responses. Together, they play crucial roles in regulating immune cell functions and maintaining immune homeostasis. CD32B and CD32C share almost identical extracellular domain. The aa sequence of Fc gamma RIIB and Fc gamma RIIC are the same for the region expressed (Ala46-Pro217)
