Human IgG1-Fc multi-tag protein final product on SDS-PAGE under reducing (P+) conditions. The purity of this protein appears to be greater than 85%. Smaller band below 30 kD might correspond to the early translational termination product.
Anti-FLAG (clone BR20M05) antibody is coated at 1 ug/mL (100ng/well). Anti-FLAG antibody can capture the multi-tag protein. HRP anti-His tag antibody (clone BR20M03) is used as the detection reagent. In the sandwich ELISA assay.
Cat #
Size
Price
Quantity
600401
25 ug
$79
600402
100 ug
$199
Product Details
Application
ELISA
Format
Liquid, Purified
Expression Host
HEK293
Target Name
Human IgG1-Fc with multiple tags (hIgG1-Fc-Myc-Avi-V5-DYKDDDDK-HA-His)
Species
Human
accession number
P01857
Sources
Human IgG1-Fc (Thr106-Lys330) protein with multiple tags (Myc-Avi-V5-FLAG-HA-His) at C-terminus is expressed in HEK293 cells.
Molecular Weight
This protein has a predicted molecular weight of 35.8 kDa. Under DTT-reducing conditions, the protein migrates at approximately 40 kDa on SDS-PAGE. The smaller band below 30 kDa might correspond to the early translational termination product.
Affinity Tag
C-Myc-Avi-V5-DYKDDDDK-HA-His
Purity
>85% based on SDS-PAGE under reducing condition
Formulation
1xPBS with 0.09% NaN3 buffer, pH7.4, 0.22 µm filtered
Endotoxin level
Not tested
Protein Concentration
25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and Handling
Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.
Background Information
Fc fragments consist of the carboxy-terminal halves of both immunoglobulin heavy chains connected by disulfide bonds, forming the constant region responsible for antibody effector functions. These functions include complement fixation, binding to Fc receptors on immune cells (such as CD16, CD32, and CD64), and mediating placental transport of antibodies. The Fc region plays a critical role in immune defense by triggering processes like opsonization, antibody-dependent cellular cytotoxicity (ADCC), and inflammation. Additionally, proteins like Protein A and Protein G, found on certain bacteria, specifically bind to the Fc region and are widely used in biotechnology for antibody purification. Recombinant Fc fragments also have potential therapeutic applications as anti-inflammatory agents in autoimmune diseases.
Human IgG1-Fc multi-tag protein final product on SDS-PAGE under reducing (P+) conditions. The purity of this protein appears to be greater than 85%. Smaller band below 30 kD might correspond to the early translational termination product.
Anti-FLAG (clone BR20M05) antibody is coated at 1 ug/mL (100ng/well). Anti-FLAG antibody can capture the multi-tag protein. HRP anti-His tag antibody (clone BR20M03) is used as the detection reagent. In the sandwich ELISA assay.
