Purified HexB (C-His) final product on SDS-PAGE under non-reducing (P-) and reducing (P+) conditions. The purity of HexB appears to be greater than 95%.
Cat #
Size
Price
Quantity
603301
25 ug
$145
603302
100 ug
$295
Product Details
Application
ELISA
Format
Liquid, Purified
Expression Host
HEK293
Target Name
Hexosaminidase B
Species
Human
accession number
P07686
Sources
Human HexB (Ala43-Met556) protein with C-terminus His tag is expressed in HEK293 cells.
Molecular Weight
This protein has a predicted molecular weight of 60.1 kDa. Under DTT-reducing conditions, the protein migrates at approximately 65 kDa on SDS-PAGE.
Affinity Tag
C-His
Purity
>95% based on SDS-PAGE under reducing condition
Formulation
25 mM Tris, 150 mM NaCl, pH 7.5 (0.2 μm filtered)
Endotoxin level
Not tested
Protein Concentration
25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and Handling
Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered 25 mM Tris, 150 mM NaCl, pH 7.5. For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.
Background Information
Beta-hexosaminidases are lysosomal enzymes that hydrolyze terminal N-acetyl-D-hexosamine residues from GM2 gangliosides and globo-sphingolipids. They exist in three isoforms: Hex A (αβ), Hex B (ββ), and Hex S (αα), formed by different combinations of α and β subunits encoded by the HEXA and HEXB genes. Recombinant HexB corresponds to Hex B isoform.
Purified HexB (C-His) final product on SDS-PAGE under non-reducing (P-) and reducing (P+) conditions. The purity of HexB appears to be greater than 95%.
