HexA-His/HexB-FLAG Heterodimer Protein

Cat # Size Price Quantity
60100125 ug$245
601002100 ug$595

Product Details


ApplicationELISA
FormatLiquid, Purified
Expression HostCHO
Target NameHexosaminidase A, Hexosaminidase B
SpeciesHuman
accession numberP07686/P06865
SourcesRecombinant human HexB (Ala43-Met556) with C-terminus DYKDDDDK tag and human HexA (Leu23-Thr529) with C-terminus His tag are co-expressed in CHO cells. Heterodimer protein is purified sequentially by anti-FLAG tag resin and Nickel resin.
Molecular WeightHuman HexA protein has the predicted molecular weight of 60 kD and human HexB protein has the predicted molecular weight of 59.8 kD . Under DTT-reducing conditions, both proteins migrate at approximately 65 kDa on SDS-PAGE.
Affinity TagC-His and C-DYKDDDDK
Purity>95% based on SDS-PAGE under reducing condition
Formulation25 mM Tris, 150 mM NaCl, pH 7.5 (0.2 μm filtered)
Endotoxin levelNot tested
Protein Concentration25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and HandlingBriefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered 25 mM Tris, 150 mM NaCl, pH 7.5. For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.

Background Information


Beta-hexosaminidases are lysosomal enzymes that hydrolyze terminal N-acetyl-D-hexosamine residues from GM2 gangliosides and globo-sphingolipids. They exist in three isoforms: Hex A (αβ), Hex B (ββ), and Hex S (αα), formed by different combinations of α and β subunits encoded by the HEXA and HEXB genes. Recombinant HexA-His/HexB-FLAG Heterodimer corresponds to Hex A isoform.