Biotinylated HexA/B heterodimer (HexA-His/HexB-FLAG) final product on SDS-PAGE under non-reducing (P-) and reducing (P+) conditions. The purity of HexA/B heterodimer (HexA-His/HexB-FLAG) is greater than 95%. Based on Gel shift Assay by co-incubation with Streptavidin, biotinylation efficiency is >95% for HexA/B heterodimer
Cat #
Size
Price
Quantity
601003
25 ug
$245
601004
100 ug
$595
Product Details
Application
ELISA
Format
Liquid, Biotinylated
Expression Host
CHO
Target Name
Hexosaminidase A, Hexosaminidase B
Species
Human
accession number
P07686/P06865
Sources
Recombinant human HexB (Ala43-Met556) with C-terminus DYKDDDDK tag and human HexA (Leu23-Thr529) with C-terminus His tag are co-expressed in CHO cells. Heterodimer protein is purified sequentially by anti-FLAG tag resin and Nickel resin. The purified heterodimer is then Biotinylated in vitro.
Molecular Weight
Human HexA protein has the predicted molecular weight of 60 kD and human HexB protein has the predicted molecular weight of 59.8 kD . Under DTT-reducing conditions, both proteins migrate at approximately 65 kDa on SDS-PAGE.
25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and Handling
Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS. For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.
Background Information
Beta-hexosaminidases are lysosomal enzymes that hydrolyze terminal N-acetyl-D-hexosamine residues from GM2 gangliosides and globo-sphingolipids. They exist in three isoforms: Hex A (αβ), Hex B (ββ), and Hex S (αα), formed by different combinations of α and β subunits encoded by the HEXA and HEXB genes. Recombinant HexA-His/HexB-FLAG Heterodimer corresponds to Hex A isoform.
Biotinylated HexA/B heterodimer (HexA-His/HexB-FLAG) final product on SDS-PAGE under non-reducing (P-) and reducing (P+) conditions. The purity of HexA/B heterodimer (HexA-His/HexB-FLAG) is greater than 95%. Based on Gel shift Assay by co-incubation with Streptavidin, biotinylation efficiency is >95% for HexA/B heterodimer
