SARS-CoV-2 Spike RBD Protein (C-Fc, Catalog # 602501) is coated at 2 µg/mL (200 ng/well). Biotinylated human ACE2 with C-Avi-His can bind RBD in the dose dependent manner. The EC50 is in the range of 5-20 ng/mL
Cat #
Size
Price
Quantity
601103
25 ug
$245
601104
100 ug
$595
Product Details
Application
ELISA, BLI
Format
Liquid, Biotinylated
Expression Host
CHO
Target Name
ACE2, angiotensin converting Enzyme 2, ACEH
Species
Human
accession number
Q9BYF1
Sources
Human ACE2 protein (Gln18-Ser740) with C-terminus His-Avi tag is expressed in CHO cells. This protein was site-specifically labeled with Biotin by BirA ligase.
Molecular Weight
This protein has a predicted molecular weight of 87.4 kDa. Under DTT-reducing conditions, the protein migrates at approximately 90-100 kDa on SDS-PAGE.
Affinity Tag
C-His-Avi
Purity
>95% based on SDS-PAGE under reducing condition
Formulation
1xPBS buffer, pH7.4, 0.22 µm filtered
Endotoxin level
Not tested
Protein Concentration
25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and Handling
Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.
Background Information
Angiotensin-converting enzyme 2 (ACE2) is a type I transmembrane zinc metalloprotease with about 60% homology to ACE, consisting of 805 amino acids including a signal peptide, catalytic domain, membrane anchor, and cytoplasmic tail. ACE2 regulates the renin-angiotensin system by converting angiotensin I to angiotensin 1-9 and angiotensin II to the vasodilator angiotensin 1-7, thus counteracting vasoconstriction and playing a key role in cardiovascular and renal function. ACE2 also serves as the functional receptor for human coronaviruses SARS-CoV, SARS-CoV-2, and HCoV-NL63, facilitating viral entry. Its expression is high in lung, heart, kidney, intestine, testis, and vascular cells. Beyond cardiovascular regulation, ACE2 is involved in respiratory disease pathogenesis, including acute respiratory distress syndrome, and has been linked to diabetes and hypertension. Additionally, its homolog collectrin is implicated in amino acid transport and genetic disorders like Hartnup disease.
SARS-CoV-2 Spike RBD Protein (C-Fc, Catalog # 602501) is coated at 2 µg/mL (200 ng/well). Biotinylated human ACE2 with C-Avi-His can bind RBD in the dose dependent manner. The EC50 is in the range of 5-20 ng/mL
