Purified Human GM2A (His24-Ile193, with C-terminus DYKDDDDK tag, 293 expressed) final product on SDS-PAGE under reducing (P+) conditions. The purity of Human GM2A appears to be greater than 95%.
Cat #
Size
Price
Quantity
601601
25 ug
$195
601602
100 ug
$395
Product Details
Application
ELISA, BLI
Format
Liquid, Purified
Expression Host
HEK293
Target Name
GM2A, Ganglioside GM2 activator
Species
Human
accession number
P17900
Sources
Recombinant human GM2A (Ser32-Ile193) with C-terminus DYKDDDDK tag was expressed in 293 cells.
Molecular Weight
This protein has a predicted molecular weight of 18.9 kDa. Under DTT-reducing conditions, the protein migrates at approximately 20 kDa on SDS-PAGE.
Affinity Tag
C-DYKDDDDK
Purity
>95% based on SDS-PAGE under reducing condition
Formulation
1xPBS with 300mM NaCl, pH7.4, 0.22 µm filtered
Endotoxin level
Not tested
Protein Concentration
25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and Handling
Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.
Background Information
GM2 ganglioside activator (GM2A) is a lipid transfer protein of the ML domain family that facilitates the degradation of ganglioside GM2 by acting as a substrate-specific cofactor for β-hexosaminidase A. GM2A extracts GM2 from membranes and presents it in soluble form to β-hexosaminidase A for cleavage, enabling conversion to GM3. It can bind several single-chain phospholipids and fatty acids and shows some calcium-independent phospholipase activity. Mutations in GM2A cause GM2-gangliosidosis type AB, a Tay-Sachs disease variant characterized by impaired GM2 degradation and lipid accumulation.
Purified Human GM2A (His24-Ile193, with C-terminus DYKDDDDK tag, 293 expressed) final product on SDS-PAGE under reducing (P+) conditions. The purity of Human GM2A appears to be greater than 95%.
