Anti-SARS-CoV2 spike antibody (clone 5D9 from Novoproteins ) is coated at 2 µg/mL (200 ng/well). Biotinylated SARS-CoV-2 Spike RBD Protein (C-His-Avi) can bind this antibody in the dose dependent manner.
Recombinant Human ACE2-His Tag protein (catalog # 600001) is coated at 2ug/mL (200ng/well). Biotinylated SARS-CoV-2 Spike RBD Protein (C-His-Avi) can bind human ACE2 in the dose-dependent manner (Quality tested).
Cat #
Size
Price
Quantity
602403
25 ug
$245
602404
100 ug
$595
Product Details
Application
ELISA, BLI
Format
Liquid, Biotinylated
Expression Host
HEK293
Target Name
SARS-CoV2 RBD, Spike RBD Protein, RBD Protein
Species
SARS-CoV-2
accession number
QHD43416.1
Sources
Recombinant SARS-CoV-2 S protein RBD (Arg319-Phe541) with C-terminus His-Avi tag was expressed in 293 Cells. This protein was site-specifically labeled with Biotin by BirA ligase.
Molecular Weight
This protein has a predicted molecular weight of 28.6 kDa. Under DTT-reducing conditions, the protein migrates at approximately 30-35 kDa on SDS-PAGE.
Affinity Tag
C-His-Avi
Purity
>95% based on SDS-PAGE under reducing condition
Formulation
1xPBS buffer, pH7.4, 0.22 µm filtered
Endotoxin level
Not tested
Protein Concentration
25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and Handling
Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.
Background Information
The receptor-binding domain (RBD) of SARS-CoV-2 is a critical region of the spike (S) protein that directly interacts with the human ACE2 receptor to facilitate viral entry into host cells. It is located within the S1 subunit of the spike protein and adopts a compact, globular structure stabilized by disulfide bonds. Due to its essential role in viral entry, the RBD is a primary target for neutralizing antibodies, vaccines, and therapeutic interventions. Mutations in the RBD—seen in various SARS-CoV-2 variants—can increase binding affinity to ACE2 or help evade immune responses.
Anti-SARS-CoV2 spike antibody (clone 5D9 from Novoproteins ) is coated at 2 µg/mL (200 ng/well). Biotinylated SARS-CoV-2 Spike RBD Protein (C-His-Avi) can bind this antibody in the dose dependent manner.
Recombinant Human ACE2-His Tag protein (catalog # 600001) is coated at 2ug/mL (200ng/well). Biotinylated SARS-CoV-2 Spike RBD Protein (C-His-Avi) can bind human ACE2 in the dose-dependent manner (Quality tested).
