Contact

GST Protein with multiple tags

GST Protein with multiple tags on SDS-PAGE under reducing conditions. The gel was stained for 1 hour with BlinkBlue Protein Staining Buffer (Catalog 700102). The purity of this protein appears to be greater than 90%. Some low molecular weight bands below 30 kDa were observed, which may correspond to early translational termination products.
GST Protein with multiple tags on SDS-PAGE under reducing conditions. The gel was stained for 1 hour with BlinkBlue Protein Staining Buffer (Catalog 700102). The purity of this protein appears to be greater than 90%. Some low molecular weight bands below 30 kDa were observed, which may correspond to early translational termination products.
Two different lots of GST Protein with multiple tags are coated at 100 ng/mL (10ng/well). Anti-His tag antibody (clone BR20M03, catalog # 300601) can detect the His tagged protein. For ELISA assay this antibody can be used at 0.1ug/mL as the detection antibody. Anti-mouse HRP is used here for detection.
Two different lots of GST Protein with multiple tags are coated at 100 ng/mL (10ng/well). Anti-His tag antibody (clone BR20M03, catalog # 300601) can detect the His tagged protein. For ELISA assay this antibody can be used at 0.1ug/mL as the detection antibody. Anti-mouse HRP is used here for detection.
Cat # Size Price Quantity
60390125 ug$79
603902100 ug$199
603903500 ug$599
6039041 mg$749

Product Details

ApplicationELISA, BLI
FormatLiquid, Purified
Expression HostE.coli
Target NameGST with multiple tags protein (Myc-Avi-V5-DYKDDDDK-HA-His)
SpeciesSchistosoma japonicum
SourcesRecombinant GST protein with multiple tags (Myc-Avi-V5-FLAG-HA-His) at C-terminus is expressed in E.coli system.
Molecular WeightThis protein has a predicted molecular weight of 35.9 kDa. Under DTT-reducing conditions, the protein migrates at approximately 35 kDa on SDS-PAGE. The smaller band below 30 kDa might correspond to the early translational termination product.
Affinity TagC-Myc-Avi-V5-DYKDDDDK-HA-His
Purity>90% based on SDS-PAGE under reducing condition
Formulation1xPBS buffer, pH7.4, 0.22 µm filtered
Endotoxin levelNot tested
Protein Concentration25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and HandlingBriefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.