Purified Pro-aerolysin Protein on SDS-PAGE under non-reducing (P-) and reducing (P+) conditions. The gel was stained for 1 hour with BlinkBlue Protein Staining Buffer (Catalog 700102). The purity of this protein appears to be greater than 95%.
Cat #
Size
Price
Quantity
604001
25 ug
$145
604002
100 ug
$325
Product Details
Application
FC
Format
Liquid, iFlour 488
Expression Host
E.coli
Target Name
Pro-aerolysin, FLAER, aerolysin, AER
Species
Aeromonas hydrophila
Sources
Recombinant Pro-aerolysin (Ala24-Arg453) with N-Met and C-terminus His tag is expressed in E.coli system. This protein is conjugated to iFlour 488.
Molecular Weight
This protein has a predicted molecular weight of 53 kDa. Under DTT-reducing conditions, the protein migrates at approximately 55 kDa on SDS-PAGE prior to conjugation.
Affinity Tag
C-His
Purity
>95% based on SDS-PAGE under reducing condition
Formulation
1xPBS/0.05%NaN3/0.2%BSA
Endotoxin level
Not tested
Protein Concentration
25µg size is bottled at 0.3mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and Handling
Briefly centrifuge the vial upon receipt. An unopened vial may be stored at 2–8°C for up to six months.
Background Information
Pro-aerolysin is the inactive precursor of aerolysin, a pore-forming toxin produced by the bacterium Aeromonas hydrophila. It has been engineered to be non-toxic. FLAER is a fluorescently labeled derivative of pro-aerolysin and serves as a valuable reagent for detecting cells that express GPI-anchored proteins.
Purified Pro-aerolysin Protein on SDS-PAGE under non-reducing (P-) and reducing (P+) conditions. The gel was stained for 1 hour with BlinkBlue Protein Staining Buffer (Catalog 700102). The purity of this protein appears to be greater than 95%.
