Recombinant Human Annexin A5 (Met1-Asp320) with N-His-Xa tag is expressed in E.coli system. His tag is cut by Xa after purification. This protein is then conjugated to iF488.
Molecular Weight
This protein has a predicted molecular weight of 35.9 kDa. Under DTT-reducing conditions, the protein migrates at approximately 35 kDa on SDS-PAGE prior to conjugation.
Affinity Tag
None
Formulation
1xPBS buffer, pH7.4, 0.09% NaN3 with a carrier protein
Endotoxin level
Not tested
Protein Concentration
25µg size is bottled at 0.1mg/mL concentration. 100 µg size is bottled at lot specific concentration.
Storage and Handling
Briefly centrifuge the vial upon receipt. An unopened vial may be stored at 2–8°C for up to six months.
Background Information
Annexin A5 is a member of the annexin family, a group of proteins that bind negatively charged phospholipids in a calcium-dependent manner. During apoptosis, phosphatidylserine (PS) translocates to the outer leaflet of the plasma membrane. Annexin A5 specifically binds to this exposed PS, making it a widely used marker for early apoptosis. It is commonly used in combination with propidium iodide (PI) in flow cytometry or microscopy assays
