Purified Cynomolgus Monkey OX40L Protein (N-Fc) on SDS-PAGE under reducing (P+) and non-reducing (P-) conditions. The purity of the purified protein appears to be greater than 95% based on reducing condition.
Biotinylated Human OX40 (C-His-Avi) is coated at 2 ug/mL (200 ng/well). Cynomolgus Monkey OX40L (N-Fc) can bind human OX40 in a dose-dependent manner with the ED50 of 3-10 ng/mL
Recombinant Cynomolgus Monkey OX40L (Gln51-Leu183) with N-terminus Fc tag is expressed in 293 cells
Molecular Weight
This protein has a predicted molecular weight of 41.4 kDa. Under DTT-reducing conditions, the protein migrates at approximately 50 kDa on SDS-PAGE.
Affinity Tag
N-Fc
Purity
>95% based on SDS-PAGE under reducing condition
Formulation
1xPBS buffer, pH7.4, 0.22 µm filtered
Endotoxin level
Not tested
Protein Concentration
25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and Handling
Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.
Background Information
OX40 ligand (also known as CD252) is a member of the tumor necrosis factor (TNF) superfamily and serves as the natural ligand for the costimulatory receptor OX40 (CD134) on T cells. Expressed primarily on antigen-presenting cells such as B cells’ dendritic cells, OX40L plays a key role in regulating immune responses by promoting T cell activation, proliferation, survival, and memory formation. The interaction between OX40L and OX40 is critical for sustaining effective adaptive immunity and is a promising target in therapies for cancer, autoimmune diseases, and chronic inflammation.
Purified Cynomolgus Monkey OX40L Protein (N-Fc) on SDS-PAGE under reducing (P+) and non-reducing (P-) conditions. The purity of the purified protein appears to be greater than 95% based on reducing condition.
Biotinylated Human OX40 (C-His-Avi) is coated at 2 ug/mL (200 ng/well). Cynomolgus Monkey OX40L (N-Fc) can bind human OX40 in a dose-dependent manner with the ED50 of 3-10 ng/mL
