Human GITRL (TNFSF18) Protein (N-His-Avi) was biotinylated in vitro using BirA ligase. SDS-PAGE analysis under reducing (P-) conditions shows the protein has a purity greater than 95%. A gel shift assay using co-incubation with streptavidin indicates that the biotinylation efficiency of the GITRL protein exceeds 70%.
Cat #
Size
Price
Quantity
606603
25 ug
$245
606604
100 ug
$595
Product Details
Application
ELISA, BLI
Format
Liquid, Biotinylated
Expression Host
CHO
Target Name
TNFSF18, GITR Ligand, AITRL, TL6, GITRL
Species
Human
accession number
Q9UNG2
Sources
Recombinant Human TNFSF18 (Glu52-Ser177) with N-terminus His-Avi tag is expressed in CHO cells. This protein was site-specifically labeled with Biotin by BirA ligase.
Molecular Weight
This prtoein has the predicted molecular weight of 18.3 kD. Under DTT-reducing conditions, the protein migrates at approximately 20 kD on SDS-PAGE
Affinity Tag
N-His-Avi
Purity
>95% based on SDS-PAGE under reducing condition
Formulation
1xPBS buffer, pH7.4, 0.22 µm filtered
Endotoxin level
Less than 0.1 EU/µg protein as determined by the LAL method
Protein Concentration
25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and Handling
Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.
Background Information
Human GITRL (Glucocorticoid-Induced TNFR-Related Ligand), also known as TNFSF18, is a member of the tumor necrosis factor (TNF) superfamily. It is a type II transmembrane protein primarily expressed on activated antigen-presenting cells, including B cells, dendritic cells, and macrophages. GITRL binds to its receptor GITR, which is found on activated T cells, regulatory T cells (Tregs), and natural killer (NK) cells. The GITR–GITRL interaction delivers a co-stimulatory signal that promotes T cell activation, proliferation, and survival, while also modulating the suppressive activity of Tregs. This signaling pathway plays a key role in immune regulation, inflammation, and antitumor immunity.
Human GITRL (TNFSF18) Protein (N-His-Avi) was biotinylated in vitro using BirA ligase. SDS-PAGE analysis under reducing (P-) conditions shows the protein has a purity greater than 95%. A gel shift assay using co-incubation with streptavidin indicates that the biotinylation efficiency of the GITRL protein exceeds 70%.
