GST with multiple tags protein (Myc-Avi-V5-DYKDDDDK-HA-His)
Species
Schistosoma japonicum
Sources
Recombinant GST protein with multiple tags (Myc-Avi-V5-FLAG-HA-His) at C-terminus is expressed in E.coli system.
Molecular Weight
This protein has a predicted molecular weight of 35.9 kDa. Under DTT-reducing conditions, the protein migrates at approximately 35 kDa on SDS-PAGE. The smaller band below 30 kDa might correspond to the early translational termination product.
Affinity Tag
C-Myc-Avi-V5-DYKDDDDK-HA-His
Purity
>90% based on SDS-PAGE under reducing condition
Regulatory Status
RUO
Formulation
1xPBS buffer, pH7.4, 0.22 µm filtered
Endotoxin level
Not tested
Protein Concentration
25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and Handling
Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.
