Recombinant Pro-aerolysin (Ala24-Arg453) with N-Met and C-terminus His tag is expressed in E.coli system. This protein is conjugated to iFlour 488.
Molecular Weight
This protein has a predicted molecular weight of 53 kDa. Under DTT-reducing conditions, the protein migrates at approximately 55 kDa on SDS-PAGE prior to conjugation.
Affinity Tag
C-His
Purity
>95% based on SDS-PAGE under reducing condition
Regulatory Status
RUO
Formulation
1xPBS/0.05%NaN3/0.2%BSA
Endotoxin level
Not tested
Protein Concentration
25µg size is bottled at 0.3mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and Handling
Briefly centrifuge the vial upon receipt. An unopened vial may be stored at 2–8°C for up to six months.
Background Information
Pro-aerolysin is the inactive precursor of aerolysin, a pore-forming toxin produced by the bacterium Aeromonas hydrophila. It has been engineered to be non-toxic. FLAER is a fluorescently labeled derivative of pro-aerolysin and serves as a valuable reagent for detecting cells that express GPI-anchored proteins.
