The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended Usage
For flow cytometric staining, it is recommended to use 5 uL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application.
Excitation Laser
Blue Laser (488 nm) Green/Yellow laser (532/561nm)
Background Information
Lymphocyte antigen 6 complex, locus G (Ly-6G), also known as Gr-1, is a 21–25 kDa glycosylphosphatidylinositol (GPI)-anchored surface protein. In mice, it is expressed predominantly on peripheral granulocytes, especially neutrophils, and on the majority of myeloid cells in the bone marrow. Ly-6G is transiently detected on developing monocytes but is stably and highly expressed on mature neutrophils. It plays important roles in neutrophil infiltration, recruitment, and migration, and associates with β2 integrins CD11a and CD11b to regulate their expression and function. The natural ligand for Ly-6G remains unknown, and it is widely used as a specific marker for murine neutrophils in immunological studies.
