The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended Usage
For flow cytometric staining, it is recommended to use 5 uL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application.
Excitation Laser
Blue Laser (488 nm)
Background Information
CD16/32 are Fc-gamma receptors (FcγRs) expressed on a variety of immune cells, including B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. CD16 corresponds to the low-affinity Fc receptor III (FcγRIII), while CD32 corresponds to Fc receptor II (FcγRII). These receptors bind antibody-antigen immune complexes, linking innate and adaptive immunity and mediating adaptive immune responses. In research, antibodies against CD16/CD32 are commonly used to block Fc receptor-mediated interactions, preventing non-specific binding of antibodies or immunoglobulin complexes to immune cells during experiments such as flow cytometry and immunohistochemistry, thereby improving experimental accuracy.
