The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended Usage
For flow cytometric staining, it is recommended to use 5 uL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application.
Excitation Laser
Blue Laser (488 nm)
Background Information
CD53 is a 35–42 kDa type III transmembrane glycoprotein and a member of the tetraspanin family. Highly glycosylated, its apparent molecular weight decreases by ~25 kDa upon endoglycosidase F treatment. CD53 acts as a membrane organizer, forming complexes with other surface proteins to regulate signal transduction, immune cell activation, migration, and survival.
