| Cat # | Size | Price | Quantity | |
|---|---|---|---|---|
| 200905 | 25 tests | $70 | ||
| 200906 | 100 tests | $150 |
| Clone | RA3-6B2 |
|---|---|
| Application | FC |
| Reactivity | Human, Mouse |
| Format | iF488 |
| Target Name | CD45R, B220 |
| Isotype | Rat IgG2a |
| Antibody Type | Monoclonal |
| Regulatory Status | RUO |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA |
| Protein Concentration | Supplied at a lot-specific concentration. |
| Storage and Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Recommended Usage | For flow cytometric staining, it is recommended to use 5 uL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application. |
| Excitation Laser | Blue Laser (488 nm) |
CD45R, also known as B220, is an isoform of CD45 (PTPRC), a member of the protein tyrosine phosphatase (PTP) family. With a molecular weight of approximately 180–240 kDa, CD45R is a transmembrane glycoprotein expressed on B cells at all developmental stages, as well as on activated B cells, subsets of T and NK cells, and certain abnormal T cell populations involved in systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. Structurally, CD45R consists of an extracellular domain, a single transmembrane region, and two tandem intracytoplasmic catalytic domains. Functionally, CD45R plays a critical role in T cell receptor (TCR) and B cell receptor (BCR) signaling by regulating Src-family kinases, which are essential for lymphocyte activation and development. The primary ligands for CD45 include galectin-1, CD2, CD3, and CD4. CD45R is widely used as a pan-B cell marker, though CD19 may provide greater specificity for B cells.
iF488 Anti-Mouse/human CD45R/B220 TDS
