The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended Usage
For flow cytometric staining, it is recommended to use 5 uL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application.
Excitation Laser
Blue Laser (488 nm) Green/Yellow laser (532/561nm)
Background Information
CD8, also known as Lyt-2, Ly-2, or T8, is a cell surface glycoprotein that functions as a co-receptor for antigen recognition and T cell activation. It is typically expressed as a disulfide-linked heterodimer of CD8α and CD8β (CD8αβ), though CD8α can also form homodimers (CD8αα). The CD8α chain is an approximately 32–34 kDa protein of the immunoglobulin superfamily. CD8αβ heterodimers are expressed on most thymocytes and a subset of mature TCRαβ T cells, while CD8αα homodimers are found on subsets of γδ T cells, intestinal intraepithelial lymphocytes (IELs), NK cells, and some dendritic cells. CD8 binds to MHC class I molecules on antigen-presenting or target cells and, through its cytoplasmic domain association with the tyrosine kinase p56^lck, facilitates intracellular signaling events essential for T cell development, activation, and cytotoxic effector function. CD8⁺ cytotoxic T lymphocytes (CTLs) play a key role in eliminating virus-infected cells, tumor cells, and cells infected by intracellular pathogens.
