The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended Usage
For flow cytometric staining, it is recommended to use 5 uL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application.
Excitation Laser
Blue Laser (488 nm) Green/Yellow laser (532/561nm)
Background Information
CD11b, also known as integrin αM or ITGAM, is a type I transmembrane glycoprotein with a molecular weight of approximately 170 kDa under reducing conditions (165 kDa non-reducing) and contains 19 potential N-glycosylation sites. CD11b associates with CD18 (integrin β2) to form the heterodimeric integrin Mac-1 (CD11b/CD18), also referred to as αMβ2, CR3 (complement receptor 3), iC3b receptor, or Mo-1. The assembly of CD11b with CD18 is required for its surface expression and function. CD11b/CD18 is one of four integrins formed by pairing the β2 chain (CD18) with different α chains (CD11a–d). Mac-1 plays an essential role in cell adhesion, migration, and phagocytosis by binding to ligands including ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen. CD11b is highly expressed on NK cells, neutrophils, monocytes, macrophages, dendritic cells, and at lower levels on subsets of T and B lymphocytes. Through these interactions, CD11b/CD18 contributes to leukocyte trafficking, complement-mediated clearance, and modulation of innate and adaptive immune responses.
