Five different antibodies—including mouse IgG2b, rabbit IgG, and human IgG1—were purified using a one-step Protein A resin protocol. The resulting purity exceeded 95% following this single-step purification.
An Fc-tagged recombinant protein was purified using Protein A resin and sequentially eluted with 0.1 M citric acid at pH 4.0 and pH 3.0. The eluted fractions were analyzed by SDS-PAGE under reducing (+) and non-reducing (-) conditions. The pH 3.0 eluate predominantly contained aggregated protein, whereas the pH 4.0 eluate primarily contained the expected dimeric form.
An Fc-tagged recombinant protein was purified using Protein A resin and sequentially eluted with 0.1 M citric acid at pH 4.0 and pH 3.0. The eluted fractions were analyzed by analytical SEC (superdex 200) on FPLC. The pH 3.0 eluate predominantly contained aggregated protein (>90% by integrated peak area), whereas the pH 4.0 eluate primarily contained the expected dimeric form (>90% by integrated peak area).
| Cat # | Size | Price | Quantity | |
|---|---|---|---|---|
| 700201 | 1mL | $39 | ||
| 700202 | 10mL | $179 | ||
| 700203 | 50mL | $749 | ||
| 700204 | 200mL | $1699 |
| Application | This cross-linked agarose resin conjugated with recombinant Protein A is suitable for the purification of IgG antibodies or Fc-tagged recombinant proteins using either batch or column methods. It can also be used for immunoprecipitation. Chemical stability: 0.5M NaOH, 6M guanidine hydrochloride, 20% ethanol, 8M urea, commonly used aqueous buffers for protein A purification |
|---|---|
| Product Description | Cross-linked agarose conjugated with recombinant Protein A. The resin is supplied as a 50% slurry in PBS containing 20% ethanol. |
| User Manual | Protein A Resin Application Guidelines (PDF) |
| Storage and Handling | Store the vial at 4°C (DO NOT FREEZE). The unopened vial is stable for twelve months when stored at 4°C. |
