His-tagged recombinant protein was coated at 100 ng/mL (10 ng/well). Detection was performed using HRP-conjugated anti-His antibody (clone BR20M03), which demonstrated dose-dependent binding. The antibody was conjugated using the IC-Link HRP Conjugation Kit.
Cat #
Size
Price
Quantity
700401
0.2mg
$299
700402
1mg
$499
Product Details
Application
The conjugates labled with HPR conjugation kit have been shown to be extremely stable, retaining 94% activity after storage for 95 days at 37ºC when stored at a concentration of 0.5 µg/mL.
Liquid-based reagents.
Completely scalable: conjugate anywhere from 10 µg to 1 gram IgG per reaction.
Supplies sufficient activated HRP to conjugate all IgG at a 4:1 HRP:IgG ratio.
Highly efficient HRP incorporation - purification not usually necessary.
Customize the HRP:IgG ratio to create optimized conjugates for different applications.
Conjugates have greatly improved stability vs Lightning-Link™ and traditional chemistry.
Product Description
The IC-Link HRP-IgG conjugation kit utilizes a highly robust chemistry to generate highly reproducible IgG-HRP conjugates with a simple procedure.
His-tagged recombinant protein was coated at 100 ng/mL (10 ng/well). Detection was performed using HRP-conjugated anti-His antibody (clone BR20M03), which demonstrated dose-dependent binding. The antibody was conjugated using the IC-Link HRP Conjugation Kit.
