Recombinant human CD64 (Gln16–Thr287) with a C-terminal His tag is expressed in CHO cells and conjugated to APC.
accession number
P12314
Molecular Weight
The protein has a predicted molecular weight of 32kDa. Under DTT-reducing conditions, it migrates at approximately 50–60 kDa on SDS-PAGE prior to conjugation.
Affinity Tag
C-His
Formulation
1xPBS buffer, pH7.4, 0.09% NaN3 with a carrier protein
Endotoxin level
Not tested
Protein Concentration
25µg size is bottled at 0.1mg/mL concentration. 100 µg size is bottled at lot specific concentration.
Storage and Handling
Briefly centrifuge the vial upon receipt. An unopened vial may be stored at 2–8°C for up to six months.
Background Information
CD64, also known as FcγRI or FcR I, is a 72 kDa type I glycoprotein and a member of the immunoglobulin superfamily. This high-affinity IgG Fc receptor is predominantly expressed on monocytes, macrophages, dendritic cells, and activated granulocytes. Its expression can be upregulated by IFN-γ stimulation, enhancing its role in immune responses. CD64 binds IgG immune complexes and is involved in several crucial immune functions, including antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC). By mediating these processes, CD64 contributes to the activation of innate immune responses and the clearance of immune complexes.
