Recombinant Human CD20 protein with C-terminusTrxA tag is expressed in E.coli and conjugated to APC.
accession number
P11836
Molecular Weight
The protein has a predicted molecular weight of 54kDa. Under DTT-reducing conditions, it migrates at approximately 70 kDa on SDS-PAGE prior to conjugation.
Affinity Tag
C-TrxA
Formulation
1xPBS buffer, pH7.4, 0.09% NaN3 with a carrier protein
Endotoxin level
Not tested
Protein Concentration
25µg size is bottled at 0.1mg/mL concentration. 100 µg size is bottled at lot specific concentration.
Storage and Handling
Briefly centrifuge the vial upon receipt. An unopened vial may be stored at 2–8°C for up to six months.
Background Information
CD20, also known as MS4A1, B1, Bp35, and Leu-16, is a 33–37 kDa glycosylated phosphoprotein with four transmembrane domains, primarily expressed on B cells from the late pro-B stage to memory B cells, but absent on early pro-B cells, plasmablasts, and plasma cells. It is also weakly expressed on some T cells and follicular dendritic cells. CD20 plays a key role in B-cell activation and proliferation, possibly acting as a calcium channel through homo-oligomeric complexes. It interacts with Src family kinases (Lyn, Lck, Fyn) and forms complexes with MHC class I/II, CD53, CD81, and CD82. Although it lacks a known natural ligand, CD20 is essential for T-independent B-cell immune responses. It is highly expressed in B-cell malignancies such as lymphomas, CLL, and hairy cell leukemia, and is the target of therapeutic antibodies including rituximab, ibritumomab tiuxetan, and tositumomab. Mutations in CD20/MS4A1 can lead to common variable immunodeficiency type 5 (CVID5), characterized by antibody deficiency, hypogammaglobulinemia, and recurrent infections.
