Human Mesothelin (E296-G580, C-His-Avi) was biotinylated in vitro using BirA ligase. SDS-PAGE analysis under non-reducing (P−) conditions shows the protein has a purity greater than 95%. A gel shift assay using co-incubation with streptavidin indicates that the biotinylation efficiency of the Mesothelin protein exceeds 80%.
Cat #
Size
Price
Quantity
801303
25 ug
$245
801304
100 ug
$595
Product Details
Application
ELISA, BLI
Format
Liquid, Biotinylated
Expression Host
HEK293
Target Name
Mesothelin, MPF, MSLN
Species
Human
Sources
Human Mesothelin protein (Accession # AAH09272.1) (Glu296-Gly580) with C-terminus His-Avi tag is expressed in HEK293 cells. This protein was site-specifically labeled with Biotin by BirA ligase.
accession number
Q13421
Molecular Weight
The protein has a predicted molecular weight of 36kDa. Under DTT-reducing conditions, it migrates at approximately 35-45 kDa.
Affinity Tag
C-His-Avi
Purity
>95% based on SDS-PAGE under reducing condition
Formulation
1xPBS buffer, pH7.4, 0.22 µm filtered
Endotoxin level
Not tested
Protein Concentration
25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and Handling
Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.
Background Information
Mesothelin (MSLN), also known as CAK1, is a 70 kDa cell-surface protein cleaved into MPF (30 kDa) and mesothelin (40 kDa). MPF acts as a cytokine, while mesothelin is membrane-bound via glycosylphosphatidylinositol. It is overexpressed in various cancers, including mesotheliomas and ovarian cancer, and interacts with MUC16/CA-125, a cancer biomarker. Elevated mesothelin levels in serum make it a target for diagnostics and therapies, such as the recombinant immunotoxin SS1P, which has shown promise in clinical trials.
Human Mesothelin (E296-G580, C-His-Avi) was biotinylated in vitro using BirA ligase. SDS-PAGE analysis under non-reducing (P−) conditions shows the protein has a purity greater than 95%. A gel shift assay using co-incubation with streptavidin indicates that the biotinylation efficiency of the Mesothelin protein exceeds 80%.
