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Human Mesothelin (E296-G580) Protein (C-His)

Human Mesothelin (E296-G580) Protein (C-His) on SDS-PAGE under reducing condition. The gel was stained for 1 hour with BlinkBlue (catalog 700102). The purity of this protein appears to be greater than 95%.
Human Mesothelin (E296-G580) Protein (C-His) on SDS-PAGE under reducing condition. The gel was stained for 1 hour with BlinkBlue (catalog 700102). The purity of this protein appears to be greater than 95%.
Cat # Size Price Quantity
80150125 ug$145
801502100 ug$295

Product Details

ApplicationELISA, BLI
FormatLiquid, Purified
Expression HostHEK293
Target NameMesothelin, MPF, MSLN
SpeciesHuman
SourcesHuman Mesothelin protein (Accession # AAH09272.1) (Glu296-Gly580) with C-terminus His tag is expressed in HEK293 cells.
accession numberQ13421
Molecular WeightThe protein has a predicted molecular weight of 34kDa. Under DTT-reducing conditions, it migrates at approximately 35-45 kDa.
Affinity TagC-His
Purity>95% based on SDS-PAGE under reducing condition
Formulation1xPBS buffer, pH7.4, 0.22 µm filtered
Endotoxin levelNot tested
Protein Concentration25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and HandlingBriefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.

Background Information

Mesothelin (MSLN), also known as CAK1, is a 70 kDa cell-surface protein cleaved into MPF (30 kDa) and mesothelin (40 kDa). MPF acts as a cytokine, while mesothelin is membrane-bound via glycosylphosphatidylinositol. It is overexpressed in various cancers, including mesotheliomas and ovarian cancer, and interacts with MUC16/CA-125, a cancer biomarker. Elevated mesothelin levels in serum make it a target for diagnostics and therapies, such as the recombinant immunotoxin SS1P, which has shown promise in clinical trials.