CHO cells transfected with either Mesothelin CAR or Mock plasmid were stained with APC conjugated Mesothelin (C-His) protein at 4ug_test
Cat #
Size
Price
Quantity
801505
25 ug
$245
801506
100 ug
$595
Product Details
Application
FC
Format
Liquid, APC
Expression Host
HEK293
Target Name
Mesothelin, MPF, MSLN
Species
Human
Sources
Human Mesothelin protein (Accession # AAH09272.1) (Glu296-Gly580) with C-terminus His tag is expressed in HEK293 cells and conjugated to APC.
accession number
Q13421
Molecular Weight
The protein has a predicted molecular weight of 34kDa. Under DTT-reducing conditions, it migrates at approximately 35-45 kDa on SDS-PAGE prior to conjugation.
Affinity Tag
C-His
Formulation
1xPBS buffer, pH7.4, 0.09% NaN3 with a carrier protein
Endotoxin level
Not tested
Protein Concentration
25µg size is bottled at 0.1mg/mL concentration. 100 µg size is bottled at lot specific concentration.
Storage and Handling
Briefly centrifuge the vial upon receipt. An unopened vial may be stored at 2–8°C for up to six months.
Background Information
Mesothelin (MSLN), also known as CAK1, is a 70 kDa cell-surface protein cleaved into MPF (30 kDa) and mesothelin (40 kDa). MPF acts as a cytokine, while mesothelin is membrane-bound via glycosylphosphatidylinositol. It is overexpressed in various cancers, including mesotheliomas and ovarian cancer, and interacts with MUC16/CA-125, a cancer biomarker. Elevated mesothelin levels in serum make it a target for diagnostics and therapies, such as the recombinant immunotoxin SS1P, which has shown promise in clinical trials.
CHO cells transfected with either Mesothelin CAR or Mock plasmid were stained with APC conjugated Mesothelin (C-His) protein at 4ug_test
