Human Glypican 3 Protein (C-His) on SDS-PAGE under reducing condition (P+) and non-reducing condition (P-). The gel was stained for 1 hour with BlinkBlue (catalog 700102). The purity of this protein appears to be greater than 95%.
Cat #
Size
Price
Quantity
802001
25 ug
$145
802002
100 ug
$295
Product Details
Application
ELISA, BLI
Format
Liquid, Purified
Expression Host
CHO
Target Name
GPC3, GTR2-2, MXR7, SDYS, OCI5,
Species
Human
Sources
Human Glypican-3 (Gln25-His559) with C-terminus His tag is expressed in CHO cells
accession number
P51654
Molecular Weight
The protein has a predicted molecular weight of 62kDa. Under DTT-reducing conditions, it migrates at approximately 65-100 kDa on SDS-PAGE.
Affinity Tag
C-His
Purity
>95% based on SDS-PAGE under reducing condition
Formulation
1xPBS buffer, pH7.4, 0.22 µm filtered
Endotoxin level
Not tested
Protein Concentration
25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and Handling
Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.
Background Information
Glypicans are heparan sulfate proteoglycans anchored to the cell surface by a glycosyl-phosphatidylinositol (GPI) linkage. There are six known mammalian glypicans (GPC1 to GPC6), which can be released by the lipase Notum. They regulate signaling pathways like Wnt, Hedgehog, FGF, and BMPs. Glypican-3 (GPC3) plays a role in modulating IGF2 interactions and influences cell proliferation. It also interacts with FGF-basic via heparan sulfate chains. Mutations in GPC3 cause Simpson-Golabi-Behmel Syndrome (SGBS), leading to overgrowth, dysmorphism, and malformations. GPC3 is expressed as a 70 kDa precursor, cleaved into a 40 kDa N-terminal and 30 kDa C-terminal subunit. It is highly expressed in Hepatocellular carcinoma and melanoma, but downregulated in cancers like ovarian, cholangiocarcinoma, mesothelioma, and breast cancer due to promoter hypermethylation.
Human Glypican 3 Protein (C-His) on SDS-PAGE under reducing condition (P+) and non-reducing condition (P-). The gel was stained for 1 hour with BlinkBlue (catalog 700102). The purity of this protein appears to be greater than 95%.
