CHO cells transfected with either PD1 CAR or Mock plasmid were stained with APC conjugated PD1 (C-His) protein at 1ug_test
Cat #
Size
Price
Quantity
803003
25 ug
$245
803004
100 ug
$595
Product Details
Application
FC
Format
Liquid, APC
Expression Host
HEK293
Target Name
PD1, PDCD1, CD279, SLEB2
Species
Human
Sources
Human PD-1 protein (NP_005009.2) (Leu25-Gln167) with C-terminus His tag is expressed in HEK293 cells and conjugated to APC
accession number
Q15116
Molecular Weight
The protein has a predicted molecular weight of 17 kDa. Under DTT-reducing conditions, it migrates at approximately 30-45 kDa on SDS-PAGE prior to conjugation.
Affinity Tag
C-His
Formulation
1xPBS buffer, pH7.4, 0.09% NaN3 with a carrier protein
Endotoxin level
Not tested
Protein Concentration
25µg size is bottled at 0.1mg/mL concentration. 100 µg size is bottled at lot specific concentration.
Storage and Handling
Briefly centrifuge the vial upon receipt. An unopened vial may be stored at 2–8°C for up to six months.
Background Information
Programmed Death-1 receptor (PD-1, also known as CD279) is a type I transmembrane protein and an immunoregulatory receptor of the CD28/CTLA-4 family. It is expressed on activated T cells, B cells, monocytes, dendritic cells, and some thymocytes. PD-1 binds to ligands PD-L1 and PD-L2, transmitting co-inhibitory signals that suppress T-cell activation, proliferation, cytokine production, and cytotoxic activity by dephosphorylating key signaling molecules. This mechanism promotes immune tolerance and prevents autoimmunity but is exploited by tumors to evade immune surveillance, as many tumors upregulate PD-L1. When tumor-expressed PD-L1 engages PD-1 on immune cells, it blocks T-cell activation and promotes immune exhaustion. Monoclonal antibodies targeting the PD-1/PD-L1 pathway have revolutionized cancer therapy by releasing this immune brake, enhancing anti-tumor immunity and leading to tumor regression in many cancers. Examples include nivolumab and pembrolizumab. This immunotherapy approach is now a major focus in oncology, offering a powerful tool to boost the immune system against cancer and reshape treatment paradigms.
CHO cells transfected with either PD1 CAR or Mock plasmid were stained with APC conjugated PD1 (C-His) protein at 1ug_test
