Recombinant Human CD171/L1CAM protein (Ile20-Glu1120) with C-terminus His tag is expressed in CHO cells and conjugated to APC
accession number
P32004
Molecular Weight
The protein has a predicted molecular weight of 124.7 kDa. Under DTT-reducing conditions, it migrates at approximately 160-200 kDa on SDS-PAGE prior to conjugation.
Affinity Tag
C-His
Formulation
1xPBS buffer, pH7.4, 0.09% NaN3 with a carrier protein
Endotoxin level
Not tested
Protein Concentration
25µg size is bottled at 0.1mg/mL concentration. 100 µg size is bottled at lot specific concentration.
Storage and Handling
Briefly centrifuge the vial upon receipt. An unopened vial may be stored at 2–8°C for up to six months.
Background Information
CD171, also known as L1CAM or L1, is a 200–220 kD transmembrane glycoprotein and a member of the immunoglobulin superfamily, originally identified for its essential role in nervous system development. It mediates neuron-neuron adhesion, axon guidance, signal transduction, cell migration, and differentiation. Although initially thought to be restricted to neural tissue, L1CAM has since been detected in various non-neural tissues and numerous cancer types. Its expression in tumors is associated with increased cell motility, proliferation, treatment resistance, and poor prognosis, making it a promising target for anti-cancer therapy. Mutations in the L1CAM gene are responsible for the CRASH spectrum of X-linked neurological disorders, including corpus callosum hypoplasia, mental retardation, aphasia, spastic paraplegia, and hydrocephalus. L1CAM interacts with several ligands such as integrins, axonin-1, CD9, and neurocan, with the RGD motif in its sixth Ig domain playing a key role in integrin binding and intracellular signaling.
