Human CD7 Protein (C-His-Avi) on SDS-PAGE under reducing condition (P+) and non-reducing condition (P-). The gel was stained for 1 hour with BlinkBlue (catalog 700102). The purity of this protein appears to be greater than 95% based on reducing conditions.
Human SECTM1 (C-Fc) protein is coated at 1 μg/mL (100ng/well). Human CD7 Protein (C-His-Avi) can detect the SECTM1 protein in the dose dependent manner. The ED50 is about 10-40 ng/mL.
Cat #
Size
Price
Quantity
805401
25 ug
$145
805402
100 ug
$295
Product Details
Application
ELISA, BLI
Format
Liquid, Purified
Expression Host
CHO
Target Name
CD7, GP40, TP41, LEU-9, Tp40
Species
Human
Sources
Recombinant Human CD7 (Ala26-Pro180) with C-terminus His tag & Avi tag is expressed in CHO cell
accession number
P09564
Molecular Weight
The protein has a predicted molecular weight of 18 kDa. Under DTT-reducing conditions, it migrates at approximately 30-40 kDa on SDS-PAGE.
Affinity Tag
C-His-Avi
Purity
>95% based on SDS-PAGE under reducing condition
Formulation
1xPBS buffer, pH7.4, 0.22 µm filtered
Endotoxin level
Not tested
Protein Concentration
25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and Handling
Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.
Background Information
CD7, also known as GP40, LEU-9, and TP41, is a 40 kD transmembrane glycoprotein of the immunoglobulin superfamily. It is expressed on thymocytes, T cells, NK cells, myeloid precursor cells, and some leukemia cells. CD7 plays a key role in T cell interactions and early lymphoid development. It activates the PI3K pathway through its intracellular YEEM motif and induces mitogenesis and IL-2 production when treated with anti-CD3 or PMA. CD7 is overexpressed in classical Hodgkin lymphoma and T-cell acute lymphoblastic leukemia (T-ALL), and is a target for CAR T-cell therapies.
Human CD7 Protein (C-His-Avi) on SDS-PAGE under reducing condition (P+) and non-reducing condition (P-). The gel was stained for 1 hour with BlinkBlue (catalog 700102). The purity of this protein appears to be greater than 95% based on reducing conditions.
Human SECTM1 (C-Fc) protein is coated at 1 μg/mL (100ng/well). Human CD7 Protein (C-His-Avi) can detect the SECTM1 protein in the dose dependent manner. The ED50 is about 10-40 ng/mL.
