Human Trop2 Protein (C-Fc-His) on SDS-PAGE under reducing condition (P+) and non-reducing condition (P-). The gel was stained for 1 hour with BlinkBlue (catalog 700102). The purity of this protein appears to be greater than 95% based on reducing conditions.
Cat #
Size
Price
Quantity
806101
25 ug
$145
806102
100 ug
$295
Product Details
Application
ELISA, BLI
Format
Liquid, Purified
Expression Host
CHO
Target Name
TROP2,TACSTD2, GA733-1, M1S1
Species
Human
Sources
Recombinant Human Trop2 (Gln31-Thr274) with C-terminus Fc tag is expressed in CHO cell.
accession number
P09758
Molecular Weight
The protein has a predicted molecular weight of 54 kDa. Under DTT-reducing conditions, it migrates at approximately 65-80 kDa on SDS-PAGE.
Affinity Tag
C-Fc-His
Purity
>95% based on SDS-PAGE under reducing condition
Formulation
1xPBS buffer, pH7.4, 0.22 µm filtered
Endotoxin level
Not tested
Protein Concentration
25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and Handling
Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.
Background Information
TROP-2, also known as TACSTD2, is a 35.7 kD protein that belongs to the EpCAM family. It is a cell surface receptor that can transduce calcium signals. Mutations of this gene are associated with gelatinous drop-like corneal dystrophy. TROP-2 is highly expressed in a variety of epithelial cancers, making it a potential therapeutic target. The cytoplasmic tail of TROP-2 contains potential phosphorylation sites and a phosphatidyl-inositol binding sequence, suggesting its role in signal transduction. As a member of a family of at least two type I membrane proteins, TROP-2 is closely related to EpCAM, also known as TROP-1, and may play a role in regulating carcinoma cell growth.
Human Trop2 Protein (C-Fc-His) on SDS-PAGE under reducing condition (P+) and non-reducing condition (P-). The gel was stained for 1 hour with BlinkBlue (catalog 700102). The purity of this protein appears to be greater than 95% based on reducing conditions.
