Recombinant Human Trop1 (Gln24-Lys265) with C-terminus Fc tag is expressed in CHO cell and conjugated to PE.
accession number
P16422
Molecular Weight
The protein has a predicted molecular weight of 53.5 kDa. Under DTT-reducing conditions, it migrates at approximately 60-65 kDa on SDS-PAGE prior to conjugation.
Affinity Tag
C-Fc
Formulation
1xPBS buffer, pH7.4, 0.09% NaN3 with a carrier protein
Endotoxin level
Not tested
Protein Concentration
25µg size is bottled at 0.1mg/mL concentration. 100 µg size is bottled at lot specific concentration.
Storage and Handling
Briefly centrifuge the vial upon receipt. An unopened vial may be stored at 2–8°C for up to six months.
Background Information
Ep-CAM, also known as tumor-associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2 (EGP2), adenocarcinoma-associated antigen, and TROP1, is a 40 kD glycosylated type I transmembrane protein containing six disulfide bridges and one THYRO domain. It is highly expressed on normal epithelial cells, including those in bone marrow, colon, and lung, and is also found on carcinomas, particularly those of gastrointestinal origin. Recent studies have shown that Ep-CAM expression occurs during early erythrogenesis. As a homotypic calcium-independent cell adhesion molecule, it plays a role in carcinogenesis by inducing genes involved in cellular metabolism and proliferation. Ep-CAM has been identified as a potential immunotherapeutic target for the treatment of human carcinomas.
