Contact

PE Conjugated Human CTLA4 Protein (C-His)

CHO cells transfected with either CTLA4 CAR or Mock plasmid were stained with PE conjugated CTLA4 (C-His) protein at 1ug/test
CHO cells transfected with either CTLA4 CAR or Mock plasmid were stained with PE conjugated CTLA4 (C-His) protein at 1ug/test
Cat # Size Price Quantity
80800125 ug$245
808002100 ug$595

Product Details

ApplicationFC
FormatLiquid, PE
Expression HostCHO
Target NameCTLA4, CD152
SpeciesHuman
SourcesRecombinant Human CTLA4 Protein (Ala37-Phe162) with C-terminus His-tag is expressed in CHO cell and conjugated to PE.
accession numberP16410
Molecular WeightThe protein has a predicted molecular weight of 15 kDa. Under DTT-reducing conditions, it migrates at approximately 25 kDa on SDS-PAGE prior to conjugation.
Affinity TagC-His
Formulation1xPBS buffer, pH7.4, 0.09% NaN3 with a carrier protein
Endotoxin levelNot tested
Protein Concentration25µg size is bottled at 0.1mg/mL concentration. 100 µg size is bottled at lot specific concentration.
Storage and HandlingBriefly centrifuge the vial upon receipt. An unopened vial may be stored at 2–8°C for up to six months.

Background Information

Cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), also known as CD152, is an immune checkpoint receptor found on T cells. It shares 30% homology with CD28 and binds to the same ligands, B7.1/CD80 and B7.2/CD86, but with higher affinity. After T-cell activation, increased CTLA-4 expression leads to immune suppression by binding to CD80/CD86, inhibiting further T-cell activation through a process called trans-endocytosis.CTLA-4 is crucial for immune regulation and tolerance, especially on regulatory T cells (Tregs). Soluble CTLA-4 (sCTLA-4) further enhances Treg function and is linked to autoimmune diseases like type 1 diabetes. Additionally, CTLA-4 plays a role in chronic lymphocytic leukemia (CLL) progression.Due to its immune-modulatory role, CTLA-4 is targeted in cancer therapy. Ipilimumab, a monoclonal antibody that blocks CTLA-4, is approved for melanoma and is being explored for other cancers.