CHO cells transfected with either CTLA4 CAR or Mock plasmid were stained with PE conjugated CTLA4 (C-Fc) protein at 1ug/test
Cat #
Size
Price
Quantity
808301
25 ug
$245
808302
100 ug
$595
Product Details
Application
FC
Format
Liquid, PE
Expression Host
CHO
Target Name
CTLA4, CD152
Species
Human
Sources
Recombinant Human CTLA4 Protein (Ala37-Phe162) with C-terminus Fc-tag is expressed in CHO cell and conjugated to PE.
accession number
P16410
Molecular Weight
The protein has a predicted molecular weight of 39.7 kDa. Under DTT-reducing conditions, it migrates at approximately 50 kDa on SDS-PAGE prior to conjugation.
Affinity Tag
C-Fc
Formulation
1xPBS buffer, pH7.4, 0.09% NaN3 with a carrier protein
Endotoxin level
Not tested
Protein Concentration
25µg size is bottled at 0.1mg/mL concentration. 100 µg size is bottled at lot specific concentration.
Storage and Handling
Briefly centrifuge the vial upon receipt. An unopened vial may be stored at 2–8°C for up to six months.
Background Information
Cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), also known as CD152, is an immune checkpoint receptor found on T cells. It shares 30% homology with CD28 and binds to the same ligands, B7.1/CD80 and B7.2/CD86, but with higher affinity. After T-cell activation, increased CTLA-4 expression leads to immune suppression by binding to CD80/CD86, inhibiting further T-cell activation through a process called trans-endocytosis.CTLA-4 is crucial for immune regulation and tolerance, especially on regulatory T cells (Tregs). Soluble CTLA-4 (sCTLA-4) further enhances Treg function and is linked to autoimmune diseases like type 1 diabetes. Additionally, CTLA-4 plays a role in chronic lymphocytic leukemia (CLL) progression.Due to its immune-modulatory role, CTLA-4 is targeted in cancer therapy. Ipilimumab, a monoclonal antibody that blocks CTLA-4, is approved for melanoma and is being explored for other cancers.
CHO cells transfected with either CTLA4 CAR or Mock plasmid were stained with PE conjugated CTLA4 (C-Fc) protein at 1ug/test
