Human CD73 Protein (C-His-Avi) on SDS-PAGE under reducing condition (P+) and non-reducing condition (P-). The gel was stained for 1 hour with BlinkBlue (catalog 700102). The purity of this protein appears to be greater than 95%.
Cat #
Size
Price
Quantity
809801
25 ug
$145
809802
100 ug
$295
Product Details
Application
ELISA, BLI
Format
Liquid, Purified
Expression Host
CHO
Target Name
CD73, NT5E
Species
Human
Sources
Recombinant Human CD73 Protein (Trp27-Lys547) with C-terminus His-Avi-tag is expressed in CHO cell.
accession number
P21589
Molecular Weight
The protein has a predicted molecular weight of 61.3 kDa. Under DTT-reducing conditions, it migrates at approximately 65 kDa on SDS-PAGE.
Affinity Tag
C-His-Avi
Purity
>95% based on SDS-PAGE under reducing condition
Formulation
1xPBS buffer, pH7.4, 0.22 µm filtered
Endotoxin level
Not tested
Protein Concentration
25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and Handling
Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.
Background Information
5'-nucleotidase, also known as NT5E, NTE, or CD73, is a GPI-anchored membrane protein that belongs to the 5'-nucleotidase family. It is expressed on T and B lymphocytes and catalyzes the conversion of purine and pyrimidine nucleotides into their corresponding nucleosides. CD73 functions as a costimulatory molecule in T cell activation and plays a key role in immune responses. CD73 generates adenosine, which acts in cell signaling across various physiological systems, including the intestinal epithelium, ischemic myocardium, and cholinergic synapses. It also helps mediate lymphocyte-stromal interactions and can condition the local microenvironment for lymphocyte function. In the absence of CD73, levels of adhesion molecules like ICAM-1, VCAM-1, and E-selectin increase on cell surfaces. Additionally, CD73-produced adenosine activates G protein-coupled purinergic receptors, influencing cellular responses. It is also involved in regulating pro-inflammatory molecules in endothelial cells, further contributing to immune and inflammatory processes.
Human CD73 Protein (C-His-Avi) on SDS-PAGE under reducing condition (P+) and non-reducing condition (P-). The gel was stained for 1 hour with BlinkBlue (catalog 700102). The purity of this protein appears to be greater than 95%.
