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Protein A resin

Five different antibodies—including mouse IgG2b, rabbit IgG, and human IgG1—were purified using a one-step Protein A resin protocol. The resulting purity exceeded 95% following this single-step purification.

An Fc-tagged recombinant protein was purified using Protein A resin and sequentially eluted with 0.1 M citric acid at pH 4.0 and pH 3.0. The eluted fractions were analyzed by SDS-PAGE under reducing (+) and non-reducing (-) conditions. The pH 3.0 eluate predominantly contained aggregated protein, whereas the pH 4.0 eluate primarily contained the expected dimeric form.

An Fc-tagged recombinant protein was purified using Protein A resin and sequentially eluted with 0.1 M citric acid at pH 4.0 and pH 3.0. The eluted fractions were analyzed by analytical SEC (superdex 200) on FPLC. The pH 3.0 eluate predominantly contained aggregated protein (>90% by integrated peak area), whereas the pH 4.0 eluate primarily contained the expected dimeric form (>90% by integrated peak area).

Cat #	Size	Price	Quantity
700201	1mL	$39
700202	10mL	$179
700203	50mL	$749
700204	200mL	$1699

Product Details

Application	This cross-linked agarose resin conjugated with recombinant Protein A is suitable for the purification of IgG antibodies or Fc-tagged recombinant proteins using either batch or column methods. It can also be used for immunoprecipitation. Chemical stability: 0.5M NaOH, 6M guanidine hydrochloride, 20% ethanol, 8M urea, commonly used aqueous buffers for protein A purification
Product Description	Cross-linked agarose conjugated with recombinant Protein A. The resin is supplied as a 50% slurry in PBS containing 20% ethanol.
User Manual	Protein A Resin Application Guidelines (PDF)
Storage and Handling	Store the vial at 4°C (DO NOT FREEZE). The unopened vial is stable for twelve months when stored at 4°C.

Data Sheets

Data Sheets

Protein A Resin Application Guidelines.pdf

Protein A resin TDS