Human CD3 Protein (C-Fc-Avi) was biotinylated in vitro using BirA ligase. SDS-PAGE analysis under non-reducing (P−) conditions shows the protein has a purity greater than 95%. A gel shift assay using co-incubation with streptavidin indicates that the biotinylation efficiency of the CD3 protein exceeds 90%.
Cat #
Size
Price
Quantity
810403
25 ug
$245
810404
100 ug
$595
Product Details
Application
ELISA, BLI
Format
Liquid, Biotinylated
Expression Host
CHO
Target Name
CD3-epsilon, FLJ18683, T3E, TCRE, CD3E
Species
Human
Sources
Recombinant Human CD3 Protein ( Asp 23- Asp126) with C-terminus Fc-Avi-tag is expressed in CHO cell. This protein was site-specifically labeled with Biotin by BirA ligase.
accession number
P07766
Molecular Weight
The protein has a predicted molecular weight of 40.2 kDa. Under DTT-reducing conditions, it migrates at approximately 50 kDa on SDS-PAGE .
Affinity Tag
C-Fc-Avi
Purity
>95% based on SDS-PAGE under reducing condition
Formulation
1xPBS buffer, pH7.4, 0.22 µm filtered
Endotoxin level
Less than 0.1 EU/µg protein as determined by the LAL method
Protein Concentration
25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and Handling
Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.
Background Information
CD3ε is a critical component of the T cell receptor (TCR)-CD3 complex, a transmembrane receptor essential for antigen recognition and T cell activation. It is a single-pass type I membrane glycoprotein containing an Ig-like extracellular domain and a cytoplasmic ITAM motif, which initiates intracellular signaling upon phosphorylation by Src family kinases (e.g., LCK, FYN). The TCR-CD3 complex includes two CD3ε chains, CD3γ, CD3δ, CD3ζ, and a TCR α/β or γ/δ heterodimer. CD3ε is expressed on all mature T cells, NK T cells, and some thymocytes, playing a vital role in T cell development and immune response. Mutations in CD3E disrupt T cell maturation and can lead to severe combined immunodeficiency (SCID), as well as increased susceptibility to autoimmune diseases such as type I diabetes.
Human CD3 Protein (C-Fc-Avi) was biotinylated in vitro using BirA ligase. SDS-PAGE analysis under non-reducing (P−) conditions shows the protein has a purity greater than 95%. A gel shift assay using co-incubation with streptavidin indicates that the biotinylation efficiency of the CD3 protein exceeds 90%.
