Human CD4 Protein (C-His) on SDS-PAGE under reducing condition (P+) and non-reducing condition (P-). The gel was stained for 1 hour with BlinkBlue (catalog 700102). The purity of this protein appears to be greater than 95%.
Human CD4 Protein (C-His) is coated at 1 ug/mL (100ng/well). Anti-human CD4 antibody (Clone OKT4) can bind human CD4 protein in dose-dependent manner with the ED50 of 2-10 ng/mL
Cat #
Size
Price
Quantity
810801
25 ug
$145
810802
100 ug
$295
Product Details
Application
ELISA, BLI
Format
Liquid, Purified
Expression Host
CHO
Target Name
CD4, CD4mut, LEU3
Species
Human
Sources
Recombinant Human CD4 Protein ( Lys26-Trp390) with C-terminus His-tag is expressed in CHO cell.
accession number
P01730
Molecular Weight
The protein has a predicted molecular weight of 42.3 kDa. Under DTT-reducing conditions, it migrates at approximately 55 kDa on SDS-PAGE.
Affinity Tag
C-His
Purity
>95% based on SDS-PAGE under reducing condition
Formulation
1xPBS buffer, pH7.4, 0.22 µm filtered
Endotoxin level
Not tested
Protein Concentration
25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and Handling
Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.
Background Information
CD4 is a single-pass type I transmembrane glycoprotein belonging to the immunoglobulin superfamily, composed of one Ig-like V-type and three Ig-like C2-type domains. It is expressed on helper T cells, regulatory T cells, monocytes, macrophages, and dendritic cells, functioning as a co-receptor for the T-cell receptor (TCR) by binding MHC class II molecules on antigen-presenting cells to enhance TCR signaling. CD4 also serves as the primary receptor for HIV-1, binding to viral gp120 to mediate viral entry. HIV-1 viral protein U (VpU) binds to the membrane-proximal region of CD4’s cytoplasmic domain, promoting its degradation in the endoplasmic reticulum and preventing surface expression, aiding viral immune evasion. CD4 plays a central role in T-cell activation and HIV infection and is a key focus in immunological and virological research.
Human CD4 Protein (C-His) on SDS-PAGE under reducing condition (P+) and non-reducing condition (P-). The gel was stained for 1 hour with BlinkBlue (catalog 700102). The purity of this protein appears to be greater than 95%.
Human CD4 Protein (C-His) is coated at 1 ug/mL (100ng/well). Anti-human CD4 antibody (Clone OKT4) can bind human CD4 protein in dose-dependent manner with the ED50 of 2-10 ng/mL
