Recombinant Cynomolgus CD25 (Glu22-Arg213) with C-terminus His-tag is expressed in CHO cell and conjugated to APC.
accession number
P63309
Molecular Weight
The protein has a predicted molecular weight of 23.5 kDa. Under DTT-reducing conditions, it migrates at approximately 35 kDa on SDS-PAGE prior to conjugation.
Affinity Tag
C-His
Formulation
1xPBS buffer, pH7.4, 0.09% NaN3 with a carrier protein
Endotoxin level
Not tested
Protein Concentration
25µg size is bottled at 0.1mg/mL concentration. 100 µg size is bottled at lot specific concentration.
Storage and Handling
Briefly centrifuge the vial upon receipt. An unopened vial may be stored at 2–8°C for up to six months.
Background Information
CD25, or IL-2 receptor alpha chain (IL2RA), is a type I transmembrane glycoprotein expressed on activated T cells and regulatory T cells. It binds IL-2 with low affinity on its own but forms a high-affinity receptor complex with the IL-2 receptor beta and gamma chains, triggering intracellular signaling pathways like MAPK and JAK/STAT. While CD25 is considered an activation marker on dendritic cells, they do not express the beta-chain necessary for IL-2 signaling. CD25 is involved in immune responses and is linked to autoimmune diseases like type 1 diabetes (T1D). Additionally, elevated soluble IL-2 receptor alpha (sIL-2Ralpha) levels are found in hematological malignancies, such as leukemias and lymphomas, as well as solid tumors, including melanoma and carcinomas of the kidney, head and neck, esophagus, and lung, reflecting tumor burden and activity.
