InnoCyto - Product

Biotinylated Human Mesothelin (E296-G580) Protein (C-Fc-Avi)

Human Mesothelin (E296-G580, C-Fc-Avi) was biotinylated in vitro using BirA ligase. SDS-PAGE analysis under non-reducing (P−) conditions shows the protein has a purity greater than 95%. A gel shift assay using co-incubation with streptavidin indicates that the biotinylation efficiency of the Mesothelin protein exceeds 90%.

Cat #	Size	Price	Quantity
801703	25 ug	$245
801704	100 ug	$595

Product Details

Application	ELISA, BLI
Format	Liquid, Biotinylated
Expression Host	HEK293
Target Name	Mesothelin, MPF, MSLN
Species	Human
Sources	Human Mesothelin protein (Accession # AAH09272.1) (Glu296-Gly580) with C-terminus Fc-Avi tag is expressed in HEK293 cells. This protein was site-specifically labeled with Biotin by BirA ligase.
Accession Number	Q13421
Molecular Weight	The protein has a predicted molecular weight of 60kDa. Under DTT-reducing conditions, it migrates at approximately 65 kDa.
Affinity Tag	C-Fc-Avi
Purity	>95% based on SDS-PAGE under reducing condition
Regulatory Status	RUO
Formulation	1xPBS buffer, pH7.4, 0.22 µm filtered
Endotoxin level	Not tested
Protein Concentration	25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and Handling	Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability.

Background Information

Mesothelin (MSLN), also known as CAK1, is a 70 kDa cell-surface protein cleaved into MPF (30 kDa) and mesothelin (40 kDa). MPF acts as a cytokine, while mesothelin is membrane-bound via glycosylphosphatidylinositol. It is overexpressed in various cancers, including mesotheliomas and ovarian cancer, and interacts with MUC16/CA-125, a cancer biomarker. Elevated mesothelin levels in serum make it a target for diagnostics and therapies, such as the recombinant immunotoxin SS1P, which has shown promise in clinical trials.

Data Sheets

Biotinylated Human Mesothelin (E296-G580) Protein (C-Fc-Avi) TDS