The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended Usage
For flow cytometric staining, it is recommended to use less than 0.05 ug of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application
Background Information
CD45RA is a 205–220 kDa isoform of the CD45 tyrosine phosphatase, expressed on naïve/resting T cells, medullary thymocytes, B cells, and monocytes. It is found on ~40–50% of peripheral CD4⁺ T cells, ~50% of CD8⁺ T cells, and subsets of B cells and monocytes. CD45RA enhances TCR and BCR signaling and associates with molecules like LPAP, CD1–CD4, and galectin-1. Its expression is regulated by cytokines and can be present on some activated T cells. CD45RA-specific antibodies (e.g., HI100) are widely used to identify naïve T cells and study CD4⁺ suppressor/inducer subsets.
