| Cat # | Size | Price | Quantity | |
|---|---|---|---|---|
| 300603 | 25 ug | $90 | ||
| 300604 | 100 ug | $275 |
| Clone | BR20M03 |
|---|---|
| Application | ELISA, WB, FC |
| Reactivity | His-tag, All Species Expected |
| Format | Biotin |
| Target Name | His Tag, histidine-tag |
| Isotype | Mouse IgG1 |
| Antibody Type | Monoclonal |
| Regulatory Status | RUO |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA |
| Protein Concentration | 0.2 mg/mL |
| Storage and Handling | Quick spin the vial after receiving. The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Recommended Usage | For ELISA applications, this antibody can be used at 0.5-1.0 ug/mL as the detection antibody. However, optimization by titration is suggested for best performance in each specific application. |
This antibody is raised against a synthetic 6xHis peptide. It can be used for Western blotting to detect His-tagged proteins (6xHis or more) at the N-terminus, C-terminus, or within the protein sequence. It is also suitable for flow cytometry or immunofluorescent staining of His-tagged proteins.
Biotin Anti-His tag antibody TDS