Cat # | Size | Price | Quantity | |
---|---|---|---|---|
300603 | 25 ug | $90 | ||
300604 | 100 ug | $275 |
Clone | BR20M03 |
---|---|
Application | ELISA, WB, FC |
Reactivity | His-tag, All Species Expected |
Format | Biotin |
Target Name | His Tag, histidine-tag |
Isotype | Mouse IgG1 |
Antibody Type | Monoclonal |
Regulatory Status | RUO |
Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA |
Protein Concentration | 0.2 mg/mL |
Storage and Handling | Quick spin the vial after receiving. The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
Recommended Usage | For ELISA applications, this antibody can be used at 0.5-1.0 ug/mL as the detection antibody. However, optimization by titration is suggested for best performance in each specific application. |
This antibody is raised against a synthetic 6xHis peptide. It can be used for Western blotting to detect His-tagged proteins (6xHis or more) at the N-terminus, C-terminus, or within the protein sequence. It is also suitable for flow cytometry or immunofluorescent staining of His-tagged proteins.
Biotin Anti-His tag antibody TDS