Recombinant proteins of various sizes, with either C-terminal or N-terminal His tags, were efficiently purified using Nickel resin and eluted with 400 mM imidazole in 20 mM Tris, 300 mM NaCl, pH 8.0. Eluted proteins were analyzed by SDS-PAGE under both non-reducing (P–) and reducing (P+) conditions and stained with BlinkBlue SDS-PAGE Staining Buffer for 30 minutes. The protein ladder (from top to bottom) corresponds to 185, 115, 80, 65, 50, 30, and 25 kDa, respectively.
Cat #
Size
Price
Quantity
700301
10mL
$99
700302
50mL
$399
Product Details
Application
Nickel resin is suitable for purifying recombinant proteins with a His tag (minimum 6×His) at either the N- or C-terminus. In principle, it may also bind proteins with internal His tags, though this application has not been validated by the manufacturer. The tested binding capacity is approximately 10–20 mg of His-tagged protein per mL of resin. When starting from culture supernatant, >95% purity can typically be achieved with a single-step purification using Nickel resin.
Product Description
Nickel charged cross-linked 6% agarose. The Nickel resin is supplied as 50% slurry in 20% Ethanol.
User Manual
Nickel Resin Application Guideline (PDF)
Storage and Handling
Store the vial at 4°C (DO NOT FREEZE). The unopened vial is stable for twelve months when stored at 4°C.
Recombinant proteins of various sizes, with either C-terminal or N-terminal His tags, were efficiently purified using Nickel resin and eluted with 400 mM imidazole in 20 mM Tris, 300 mM NaCl, pH 8.0. Eluted proteins were analyzed by SDS-PAGE under both non-reducing (P–) and reducing (P+) conditions and stained with BlinkBlue SDS-PAGE Staining Buffer for 30 minutes. The protein ladder (from top to bottom) corresponds to 185, 115, 80, 65, 50, 30, and 25 kDa, respectively.
